FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPHK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPHK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514006
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATG8750.060.3972936
GGATAGG17550.059.228116
GATAGGT15500.058.9316677
CGGATAG8350.058.685
GGATAGT13550.058.6320576
CGATAGG8600.057.3811456
GCCGATA6050.057.2701574
CGCTACA3050.056.2269826
GCGATAC2900.055.5147485
GGGATAG17150.055.5077135
GATAGGG14600.055.3731277
TGGATAG16500.054.936935
TGATAGG15500.054.8685076
GATAGTC7600.054.799087
CTGATAG9850.054.7183535
CGTGATA8650.054.6218264
CGATACG2500.054.5975536
CCTATAC4350.053.9056245
TGCGATA8900.053.8739854
CCGATAC1950.053.8437355
Produced by FastQC (version 0.11.5)