FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPHK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPHK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4080931
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG25450.063.260915
TGATAGG35000.061.9996226
CAGATAG27500.061.8541645
CGGATAG16050.059.7504125
CACGATA13450.059.0695464
GATAGGT21700.059.0290037
GCTAGCG21350.059.0131387
CCTGATA12900.058.3322644
GATAGTC15700.058.1815077
TTATAGC30550.058.0847556
CTATAGG17750.057.379936
CCGCTAC4900.057.14255
GATAGGA39750.057.053467
GGATAGG21000.056.9996536
CGATAGT15050.056.511286
CCGATAA10600.056.4619145
CCTATAG11500.056.3040055
GGATAGT23300.056.02976
TATAGGC22350.055.9029547
GCTACGC5950.055.8792727
Produced by FastQC (version 0.11.5)