FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPHK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPHK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2698110
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA6000.061.829984
CGGATAG8750.059.196795
GGATAGG19300.058.3906146
GCGGTCC1500.058.330175
GGATAGT18150.057.269626
CCTGATA6850.056.712254
GATAGGG18700.056.706057
TGATAGG18600.054.943266
CTGATAG10300.054.7057725
GCCGATA5650.054.5103154
CAGATAG11950.054.4740335
GATAGGT20900.054.4208647
GCGGTAC2650.054.1480065
GTGATAG18250.053.8874865
CGATAGT9500.053.7865566
GCGGATA10700.053.641954
CGATAGG9600.053.5908436
GGGATAG20050.053.5881165
AGGATAG14000.052.9971245
GCGATAG8600.052.9041065
Produced by FastQC (version 0.11.5)