FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_LPHK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_LPHK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4438390
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTC16900.060.0555847
GGATAGG24800.059.13026
CTGATAG27500.058.924425
CAGATAG26400.058.8607835
GCTAGCG19000.057.4702537
TGATAGG39450.057.3989946
CGGGTAC3050.056.2267845
CGGATAG16500.056.2093965
GATAGGT26650.055.9441227
GATAGGA42800.055.9312367
CGATAGT16850.055.664966
CCTATAG12650.055.609955
CGTGATA16300.055.396094
CGTATAG13050.055.2463655
ACGATAG14900.055.198675
CGCGATA8950.055.1369934
CCGCTAC5150.055.0458765
GGATAGT28550.054.918536
GCGATAA14350.054.875395
TATAGGC22250.054.7383047
Produced by FastQC (version 0.11.5)