FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPLK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPLK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678026
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA5300.060.0912634
GCGATAG9400.059.1990975
CGGGATA10600.059.1007464
CGGATAG8100.057.898275
CGATAGT8950.057.483096
CAGATAG12800.056.8720865
CGATAGG10050.056.415026
GCCGATA5850.055.0399134
CGGCTAC3500.054.997185
CGTATAC2700.054.4416545
CCGATAA7850.053.9462785
GGATAGT17450.053.7508246
CGATATG11650.053.473666
TGATAGG18050.053.321376
GATAGGT18550.053.2068067
GGTAGGC4300.052.9062427
CCTGATA7050.052.621424
GATAGTC10450.052.5830047
CGATAAT12250.052.2830356
CGTGATA9650.052.2253044
Produced by FastQC (version 0.11.5)