FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPLK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPLK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681336
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATAG15700.060.632375
CGCGATA4450.058.197864
GGATAGG15950.058.146096
CGGATAG9900.057.9754265
CTATAGG9700.057.366816
GATAGGT18100.056.653197
TGATAGG20650.055.9279866
CTGATAG14950.054.7784735
GGATAGT18350.054.737026
GCGATAA9300.054.1894655
CCGCTAC3100.054.189465
GCTCCCG2850.054.031017
GATAGGG15800.053.8250587
GATAGTC9600.053.589717
CCAGATA10200.053.5253754
GATAGGA20750.053.1285257
CGGGATA8050.053.0394824
GGGATAG15400.052.950555
GCGCTAC2850.052.8030325
CGCTACA5050.052.6692926
Produced by FastQC (version 0.11.5)