FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPLK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPLK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3511468
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA6500.060.306374
CGGATAG10450.059.950845
CGGGATA12550.057.170064
GATAGTC11700.057.134687
CGATAGG11650.057.080296
GGATAGT25250.056.6918266
GCGATAA12750.055.9987685
GATAGGT26800.054.5875747
CAGATAG15200.054.1106535
ACGATAG10800.053.7951165
ACGGATA9700.053.7617034
CGATAGT15050.053.48726
TGATAGG23700.053.3110666
TGCGATA14300.053.110724
GATAGGG23550.053.0554057
GCGATAG12950.052.9718135
GCGGTAC3450.052.7524645
CGGTCCC2000.052.4988486
CCTGATA10750.052.4174544
GGGATAG25700.051.886025
Produced by FastQC (version 0.11.5)