FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPLK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPLK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3397754
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG24050.061.992965
GGATAGG16300.061.6229066
TGATAGG31350.060.1726386
GATAGTC13150.060.146647
CCGCTAC3900.059.2280125
CGGATAG11350.059.2042965
GATAGGA34650.059.0855567
CGATAGT12450.059.03346
TTATAGC21250.058.9619646
GGATAGC8850.058.9238176
GCGATAG10900.058.7587365
CCGATAA9950.058.7409865
CAGATAG20700.058.668775
CTAGGAC9200.058.5807888
TCTAGCG14600.058.008447
GCTACGC5950.057.6418347
AGGATAG13300.057.6289025
GCTAGCG16950.057.60547
CCGTATA6900.057.3161774
CTATAGG15700.057.067416
Produced by FastQC (version 0.11.5)