FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPLK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPLK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216635
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10000.060.549045
GGATAGT23400.058.332416
CGATAGT11000.057.908186
CGCGATA6400.055.780374
GCGATAG11300.055.751335
CGGGATA10350.055.458064
GGGATAG22200.054.075725
TGATAGG22450.053.9412426
CAGATAG15550.053.568295
CGATAGG12100.053.5115556
GATAGGT26000.053.4389727
GATAGTG29100.053.3987277
CTGATAG13650.053.3324935
TCCGCTA3150.053.332494
ACGATAG9300.052.6873445
GGATAGG24050.051.807916
CGCTAGC3200.051.4054376
CCAGATA9000.051.3325234
CGATATG14550.051.2363056
GCGATAA12000.051.040865
Produced by FastQC (version 0.11.5)