FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPLK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPLK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3042627
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG9850.055.7821275
CGCGATA5600.055.620344
GGATAGG20900.055.4259766
GATAGGG20300.053.9601067
CCCGTCC5000.053.89282610
CGGGATA11400.052.49564
CGCTACC2800.052.4955986
GGATAGT20900.052.4118776
GCGATAA10850.052.2536855
CGATAGT11200.051.870656
GGTCCCC1900.051.5737767
CCGCTAC2900.050.6854065
GGGATAG20950.050.6162835
CAGATAG12950.050.2660565
GCGATAG10150.049.995815
GATAGGT23000.049.908047
TGATAGG24100.049.518676
CCCGATA4350.049.0763474
CCGCTAG2600.048.4574745
GATAGGA23200.048.271027
Produced by FastQC (version 0.11.5)