FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959872
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA6750.058.5914
GGATAGT21100.057.8903926
CTGATAG15850.057.8533175
CGCTAAC1600.056.874416
CGATAGT11650.056.780536
GATAGTC10300.056.4052737
CGGATAG11300.055.7506945
ACGATAG8000.055.5609865
GATAGGG21250.055.3387347
TGATAGG23950.055.0933766
GGGATAG20850.055.058455
GCGATAG11450.055.020335
CGATAGG10150.054.8270156
CGGGATA9900.054.7964864
GGATAGG21450.054.6614386
GATAGGA22750.054.6129727
CAGATAG14750.054.574785
GCGATAA10300.054.0276535
GTGATAG21050.053.7045
GATAGTG22450.053.4720277
Produced by FastQC (version 0.11.5)