FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413417
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG12050.055.4756245
CCGCTAC3000.054.8317955
GGATAGG23000.054.7810756
CGCGATA6150.054.0635264
GGATAGT27150.053.4975786
GATAGGT26250.053.4651687
GGGATAG23200.051.593385
CAGATAG16150.051.3607945
GATAGGG24900.051.3037837
CGCTACC3150.051.1096766
GCCGATA7350.050.9509544
GCGATAA12400.050.805035
CGGGATA11450.050.7409364
CTGATAG16400.050.364445
GTGATAG29800.050.2670485
CCTATAG6150.050.0798955
GCGGTAC3650.049.861615
CCCGTCC4900.049.28288710
GATAGTG33600.049.269457
CGATAGT14250.049.121436
Produced by FastQC (version 0.11.5)