FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830613
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA5450.059.0799454
GGATAGT16600.058.822696
CGGATAG9250.057.5109635
CGGGATA9950.057.3341374
CAGATAG15050.055.8114745
GATAGGT21200.053.98257
GATAGTC9800.053.9252247
TGATAGG20400.053.8701556
CTGATAG14250.053.7870835
GGGATAG17450.053.7511945
GCGATAA9850.053.652445
GATAGGA21100.052.7455377
GATAGGG17700.052.0024267
GGATAGG18950.051.7127466
CGCTACA5550.051.709416
CGATAGT11350.051.495516
ACGATAG8650.051.3850025
GCGATAG11450.051.3514335
ACGGATA9100.051.1515734
CGATAGG9800.051.069166
Produced by FastQC (version 0.11.5)