FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461959
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA4700.058.0820544
GCGATAG8450.056.3284035
GGATAGT18600.055.6966
GGATAGG17050.055.0117766
GATAGGG17700.054.9677477
GCGATAA8550.054.8509255
GATAGGT19250.054.541487
CGGATAG8900.054.266815
CGATAGT8800.054.088076
CCCGTCC2850.054.0322510
CGGGATA8450.053.8433274
ATACGGC1300.053.8433278
CGCTACC1700.053.52666
CTGATAG14100.052.373145
CAGATAG12100.052.063385
GATAGTC9150.052.0180747
TGATAGG21450.051.7222256
CCCGATA4000.050.7473344
ACGATAG8150.050.6721885
TGGATAG20600.050.6284075
Produced by FastQC (version 0.11.5)