FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048059
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT19000.056.3663336
CGGATAG8800.055.6797565
CAGATAG15750.055.55355
GATAGTC10800.055.4146167
CGCGATA6100.055.0790254
GATAGTG25600.054.822197
GATAGGA22100.054.4776237
CGGGATA11050.054.4767274
CGATAGT12500.054.317996
CGATAGG12100.054.0889056
TGATAGG20650.053.387856
GCGGTAC3150.053.3313565
CTGATAG15200.053.188825
ACGATAG10600.053.158415
GCTGATA17950.053.0333754
CCCGTCC5150.053.00687810
GCGATAA10650.052.580215
CGTGATA11100.052.3395424
GATAGGT22850.052.230017
GCGATAG12400.052.215815
Produced by FastQC (version 0.11.5)