FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_wt-0.5_HPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_wt-0.5_HPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577337
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC2500.057.398085
CGCGATA4450.055.0543334
TGATAGG22550.054.4771086
CGGCTAC3950.054.048825
GGATAGG19100.053.872546
CGGGATA8550.053.2145924
GGATAGT18400.053.0688746
CGGATAG9300.053.0627375
GCGATAA10100.053.0180245
GGGATAG17350.052.2460865
CTGATAG13850.051.8033185
GCGGTCC2100.051.6649365
GATAGGT21100.051.419087
GTGATAG22250.050.9645845
GATAGGG17150.050.8136377
GATAGTC10250.050.533917
CAGATAG11550.050.3013425
CCCGATA3900.050.2547234
CGATAGT10400.050.142556
CCTATAG5550.049.818155
Produced by FastQC (version 0.11.5)