FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9024470
Sequences flagged as poor quality0
Sequence length76
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG380890.4220635671679334No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190080.21062732769902276No Hit
GCGACCAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180700.20023336550512105No Hit
CGACCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158800.17596601240848492No Hit
AAAAAACGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110450.12238945888234988No Hit
CGACCCCGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108380.12009569537047605No Hit
CGGAAGAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104940.11628383716716882No Hit
CGGAAGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92450.10244368921388182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG188750.064.336116
TTCGCGG50350.062.901215
CGACCCG47200.062.6949772
GACCCGC44000.062.440013
TGCGCGG46400.061.3926435
ACGCGGG156150.061.3626026
GAGCGGG210200.061.0173846
CGCGGGG774050.060.9565247
AGCGGGG440150.060.7269747
TGTGGGG281800.060.1927726
AACGCGG58650.059.6083875
GTCGCGG64900.059.529785
CAGCGGG84300.059.0730866
CCGCGGG193550.058.2565426
ACCCGCG54100.057.3767974
TCCGCGG36250.057.3440485
ACCAGCG28400.056.6825564
GACCAGC28300.056.6402363
TAGCGGG90950.056.4083756
CTCGCGG46750.056.3667875