FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426480
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA8000.059.060574
CGGATAG9150.058.140185
CGCGATA5300.056.79064
GGATAGT16500.055.9981736
CGATAGG8050.055.6503566
CGATAGT9500.055.2613536
CGGCTAC3650.054.6557545
GATAGTC9800.053.9256977
GATAGTG18700.053.7137157
GCGGATA10350.053.4282074
GATAGGT19000.053.4182057
GTGATAG15350.053.3533065
GCGATAA9000.053.2760355
GCGATAG9200.053.259135
CCTGATA7450.053.085514
CGATATG9800.052.8554156
AGGATAG12300.052.3560145
GATAGGA18600.052.309047
ACGATAG7300.052.258575
CAGATAG12650.052.0141075
Produced by FastQC (version 0.11.5)