FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562921
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA5850.058.6293264
GGATAGT16150.057.643966
CGATAGG8850.056.9460876
CGATAGT9250.056.7537086
CGGGATA8900.056.6261634
CAGATAG10750.055.996995
CGGATAG10050.055.3701555
CGGCTAC4200.054.9970445
ACGATAG8200.054.631215
CGTGATA9850.054.0072484
GCGATAA10450.053.9205445
GATAGTC9700.053.7578967
GCGATAG9600.053.5908665
GATAGGT17700.053.5825967
CGCTAAC2000.052.4971736
GATAGTG18550.052.25937
CCTGATA7300.052.2574654
CGCTAGC3550.052.250716
GCGGTAC2150.052.0902185
GGTACGC1750.051.995187
Produced by FastQC (version 0.11.5)