FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3187635
Sequences flagged as poor quality0
Sequence length76
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGGG21250.058.46847
GCGGGAC601.8189894E-1258.330245
CGATAGG8350.058.2612956
GGATAGG20400.057.64496
GGATAGT25050.056.584716
CGCGATA5200.056.5354584
GATAGGT26000.056.2671287
CTGATAG14500.055.7556575
CTATAGG8200.055.0589146
GGGATAG18000.054.830425
CGGGATA8200.054.6312454
TGATAGG24400.054.5061576
GCGGTCC653.6379788E-1253.8432925
GCGATAG10750.053.7180755
CACGATA6950.053.378464
CAGATAG12450.053.129715
GGCGATA9700.053.038424
GCCGATA6550.051.829314
CGATAGT14450.051.5897646
CGCTCCC1700.051.4686626
Produced by FastQC (version 0.11.5)