FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171364
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC1700.063.820825
CCTATAG7050.060.5648085
CTGATAG12850.059.9196325
GATAGTC8250.059.3900537
CAGATAG15700.059.2968445
GGATAGG13300.058.6817176
GGATAGC5950.058.2328226
CGATAGT8000.058.185036
GATAGGT13450.057.7657367
GCTAGCG12200.057.6601567
CGCTACA2500.057.397566
CGGATAG7050.057.0897755
CGTATAG6600.056.7400175
CTATAGG9650.056.577916
CGCGATA4900.056.4261744
GCGATAG7800.056.087365
TGATAGG18100.056.074976
GATAAGC9900.055.854937
TCTAGCG8650.055.8344967
TTATAGC13300.055.7871066
Produced by FastQC (version 0.11.5)