FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPHK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPHK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872526
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA3650.060.4049224
CGGATAG9800.059.9940035
ACGATAG9700.059.1693425
CAGATAG13400.059.023955
CGATAGT9350.058.7641756
CGATAGG10150.058.270046
CCGATAA9050.058.005255
GGATAGT18600.057.7630426
GCCGATA6800.057.126644
GGGTCCC2350.056.5900846
GATAGTG20100.056.5863847
CGGGATA10000.056.3443644
GATAGGA26200.056.2348377
CCCTAGG3550.056.19156610
GCTGATA15750.055.99444
CGATATG10550.055.7290236
AGGATAG14150.055.6481445
CGTGATA10900.055.5449074
CCTGATA8800.055.2785644
CGCTACG4000.055.1194886
Produced by FastQC (version 0.11.5)