FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPHK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPHK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966104
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCG20400.060.9024547
CAGATAG24100.060.702535
CGGATAG12550.060.23625
GGATAGG21300.059.6450736
CCTGATA11300.059.4654734
CTGATAG20700.057.6542855
CGGGTCC2800.057.4972885
ATAGTGC9750.057.4317448
TTATAGC24550.057.3089036
CTATAGG14650.057.0962836
CCGATAA10250.057.02175
GATAGTC14900.056.8415227
CGATAGT14000.056.4973346
CGTGATA14150.056.1450544
GCTACGC4500.055.995947
GGATAGC11600.055.816336
GCAGATA17400.055.7150574
CCGATAC3400.055.5856135
TCTAGCG17000.055.378347
GCGATAA12900.055.3462265
Produced by FastQC (version 0.11.5)