FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPHK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPHK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285249
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA5150.057.760964
CGGATAG7950.057.666935
CGATAGT9350.057.2692536
ACGATAG6600.055.6760035
GATAGTC8300.055.659277
CGATAGG7800.055.188966
CGGGATA7400.054.859144
CCTGATA5750.054.7768864
CTGATAG11650.054.3720055
GTATAGG9350.053.9004756
GGATAGT14600.052.9762426
GATAGTT19300.052.5874827
GATAGGG14750.052.4375047
TGCGATA9350.052.4009444
GCGATAG9150.052.39895
GATAGGA17550.052.2475437
CAGATAG12500.051.7945945
TGATAGG16700.051.763336
GATAGGT17500.051.596877
CGTGATA8550.051.5735634
Produced by FastQC (version 0.11.5)