FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPHK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPHK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3133931
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGG34500.063.3000076
CTGATAG27250.063.1883285
CTATAGG17300.061.9032676
GATAGGA42250.061.462267
CCGCTAC3600.061.24585
CCTGATA9550.060.8335234
GGATAGG19400.060.79486
GCTAGCG15150.060.7539377
ATATGCG17150.060.6071248
GGGATAG15450.059.3486755
GATAGGT17350.059.3033377
CTAGGAC11750.059.271588
CCGATAA7150.059.2267045
CGGATAG12650.059.2054255
TTATAGC22400.059.0584536
GAGGACT24600.058.8974579
CAGATAG21700.058.866935
GATAGTC13500.058.587647
GCGATAA10950.058.4891365
CAGATAA18050.058.361655
Produced by FastQC (version 0.11.5)