FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPHK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPHK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534349
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG8450.058.3987585
CCTGATA6700.057.4591374
CGATAGG7500.056.463186
GGATAGT16750.056.2054866
CGGGATA9200.055.9204644
CGCGATA5200.054.5158654
GATAGTC8350.054.48557
GATAGGT18550.053.7681477
CGATATG10550.053.7407576
CGATAGT9150.053.5486076
CAGATAG12200.053.3573575
TGATAGG19750.052.984076
GTGATAG19850.052.5408365
GATAGGG16450.052.335137
CGTGATA10000.051.7968064
CGCTCCC2100.051.663486
GGATAGG19000.051.5757646
GGGATAG18950.051.527165
CTGATAG13100.051.294545
GATAGGA18650.051.228067
Produced by FastQC (version 0.11.5)