FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_LPHK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_LPHK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4091462
Sequences flagged as poor quality0
Sequence length76
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT32300.058.8372236
GGATAGG23550.058.7031026
GGGATAG22150.058.305235
CCGCTAC2650.058.1114435
CGGATAG13350.057.676155
CGGGATA10800.057.3593674
CGCGATA7550.057.0181354
GCCGATA8800.056.8732764
CTGATAG16200.056.6032185
CGCTACC2050.056.339756
GATAGGG19550.055.4963577
GATAGGT30650.055.3817
CAGATAG14350.055.3641745
GATAGTC14250.055.01527
GGTCCGC1400.054.9976587
CGGGTAC2000.054.2483525
GCGCTAC4750.053.0510185
GCGATAC3500.052.998395
CGCTCCC2250.052.887286
TGATAGG22400.052.8108986
Produced by FastQC (version 0.11.5)