FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPLK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPLK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767748
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG5950.058.8194165
CAGATAG7950.058.1091465
GGATAGT11450.058.0745436
CGATAGT6100.057.946776
GATAGTC6750.057.5499047
CGATAGG6400.056.871026
GATAGGA14450.056.6726347
CGCTACA2600.056.5345086
CGCGATA3100.056.4476664
CCGTATA3050.056.225584
GGCTACC2500.055.9960866
CCCGTCC4200.055.8278510
CGTATAG4750.055.2592965
GATAGTG11850.055.2266467
GTGATAG11900.054.9961555
CGTATAC2050.054.630335
GATAGGG12000.054.2446757
CCCGATA3750.054.1295474
AGGATAG9250.054.104335
CGTGATA6300.053.885124
Produced by FastQC (version 0.11.5)