FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPLK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPLK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901179
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA9100.057.6891254
CCCGTCC9050.056.46000710
GGATAGT20550.055.691326
CGATAGT10950.055.614336
GCGATAG11800.055.4639975
GATAGGT22250.055.2104387
TGATAGG26000.055.0556076
GGATAGG22150.054.9866266
GATAGGG21350.054.25937
GGGATAG22400.054.0604485
CGGATAG9500.053.7874345
GATAGGA26200.053.2985887
CGCGATA5400.053.1452144
TCCCGTC8700.052.6973349
CAGATAG13400.052.498015
GCGATAA9000.052.498015
ACGATAG8750.050.7980735
AGATAGG21350.049.99816
AGGATAG13950.049.9264225
GATAGTC11750.049.741947
Produced by FastQC (version 0.11.5)