FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPLK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPLK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015968
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTC1100.063.6345524
CGGGATA10850.058.7080084
CGGATAG9850.058.2724575
GGATAGT22600.057.918716
CGATAGT11300.057.918716
CGCGATA7100.057.674424
GGTCCCC1550.056.4481357
ACGATAG9450.056.2946935
GCGATAG11450.055.9372755
CGATAGG10450.055.9314236
GATAGGG19150.054.6441547
TGATAGG22400.054.3734556
GATAGGT23200.054.1561477
CCCGATA4600.054.0202034
CAGATAG12850.053.928435
CTGATAG14150.053.4260445
CGATATG13500.053.4058886
GCGATAA11600.053.4036565
GATAGTT26800.053.28037
GTGATAG22050.052.8556375
Produced by FastQC (version 0.11.5)