FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPLK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPLK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3797317
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG18750.063.6500856
CAGATAG23850.061.778825
TGATAGG35500.061.7151646
GATAGGA41500.061.5623177
CTGATAG27200.061.504225
CGGATAG12200.061.3903125
CTATAGG16100.061.0838436
GATAGTC14450.061.034157
TTATAGC28500.060.049576
GCTAGCG22950.059.168327
CGTATAG10800.058.9784775
CCGCTAC4750.058.9443665
GGGATAG16900.058.6064835
CCAGATA15150.058.4458664
GGATAGC10600.058.4404146
CCTATAG10500.058.330365
TATAGCC8050.058.2571337
CCGATAA10100.058.2148555
CGGCTAC6500.058.150885
GTAGGAC9900.057.9753158
Produced by FastQC (version 0.11.5)