FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPLK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPLK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737535
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA9900.058.6833154
GGATAGT16800.058.1214836
CGGATAG10550.057.7216225
CGCGATA6400.057.4183964
GCGATAG9950.055.926265
GCGATAA8900.055.446085
CAGATAG13800.055.286515
CGATAGG9150.054.696146
CGCTACA4000.054.2467166
GATAGGT17500.053.795767
TGATAGG19850.053.775086
CGTGATA10150.053.445044
GATAGTC10300.053.005537
CGATATG11900.052.6438756
GCCGATA6000.052.4968224
GATAGGA22300.052.4173937
TGCGATA10100.052.3235634
CGATAGT10050.052.235646
GGGTCCC2550.052.1537066
GATAGTG20400.051.98127
Produced by FastQC (version 0.11.5)