FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPLK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPLK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863293
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC2550.060.3900155
CGGGATA7950.060.3123254
GCGATAG10900.059.722665
CGCTACC2300.057.824046
GATAGGA32750.057.6010137
GATAGTC11600.057.3255587
TGATAGG28600.057.2707026
GTCCCGT3500.056.996998
TCCCGTC10350.056.808599
CGCGATA5750.056.606694
CAGATAG13400.056.415915
GTATAGG15750.056.2202266
GGATAGG18300.056.036276
GATAGGT18150.055.920887
ACGATAG9100.055.7672545
CTATAGG12950.055.6737026
CCTGATA9100.055.3826524
CGTGATA13200.055.1495634
GGATAGT20500.055.1443866
CTAGGAC7900.054.9338048
Produced by FastQC (version 0.11.5)