FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3079017
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10350.058.8378265
CAGATAG15150.057.0600365
ACGATAG8000.056.8723345
GGATAGT21100.056.72726
CGATAGT11450.056.547576
CGGGATA10200.055.5856324
TGATAGG23400.054.142766
GATAGGT22350.054.022567
GTGATAG20950.053.625175
GATAGTC11750.053.6127667
CGTGATA12300.053.2088854
GATAGGG19550.053.1671377
CGCGATA6300.052.7753034
TGGATAG22000.052.6566245
GGATAGG22050.052.537226
GCGATAA11600.052.4975365
GCGATAG11000.052.1793755
GCCGATA7200.052.011454
CTGATAG16100.051.7367065
GCTGATA16450.051.6997074
Produced by FastQC (version 0.11.5)