FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169702
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG20400.060.0476656
CCGCTAC3150.058.887565
CCTGATA8500.057.6457634
CCCGTCC9850.057.2023210
TGATAGG32150.056.608376
GATAGGA34250.056.3035247
GGATAGT24000.056.2903986
TCCCGTC9200.055.918249
GCGGTCC1950.055.639775
CTGATAG23000.055.542235
GATAGTC12500.055.4370047
CGGATAG11250.055.0654265
CAGATAG14750.055.0496065
GATAGGT20500.054.9726377
GGGATAG19000.054.5250855
CGGGATA9950.054.521394
CGCGATA6450.054.262344
GCGATAA11850.053.163365
CGTGATA13850.052.8146934
GCCGATA7850.052.610284
Produced by FastQC (version 0.11.5)