FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685516
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG9650.059.8428425
CGGGATA9900.059.7457544
GGATAGT16250.059.0136876
CGCGATA7250.057.9293674
CGATAGG8050.057.824426
GATAGTC9800.055.7116437
CGCTACA4700.055.1047976
GCGATAA9800.054.2841535
ACGATAG7900.054.0490845
GATAGGG16850.053.7956737
CGATAGT11100.052.971456
AGGATAG12900.052.6341485
GCGATAG10750.052.41715
CCGATAA8950.052.4007345
GATAGGT19050.052.3597267
GATAGGA19950.052.278237
CCTGATA7500.052.2651674
GATAGTG19900.052.2337077
GTGATAG19100.052.040395
GCTGATA14150.051.9419754
Produced by FastQC (version 0.11.5)