FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121025
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC3400.059.703715
GGATAGG20500.058.388126
GGTCCCC1500.058.3284037
CGCTACC3800.058.0242046
CGCGATA7700.057.725174
CGGGATA10100.057.176144
CCTATAG6400.056.3260775
CAGATAG13650.055.89545
GGGATAG18950.055.7763375
TCCCGTC13350.055.574059
ACGATAG9700.055.5649875
CGATAGT13100.055.303336
CGGCTCC6650.055.2611475
CGGATAG13050.055.2470325
GCGATAG12100.055.2459225
GATAGGA29450.055.1396567
TGATAGG27950.054.8459476
CCCGTCC14850.054.43810310
GATAGGT20850.054.3838967
GATAGGG20050.053.9355967
Produced by FastQC (version 0.11.5)