FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565729
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG13350.059.2462355
CGCGATA7300.058.9679224
CGCTACA5800.058.5298426
GGATAGT23400.057.5809026
CGATAGT13400.057.458136
CAGATAG17400.057.1219025
GCGATAG12200.056.7987755
GCTGATA19150.055.3741954
GATAGTG25100.055.3541767
GTGATAG23400.055.337495
CGATATG13800.055.0318686
GATAGGA30900.054.930927
CCGATAA10400.054.851425
CGGGATA12350.054.408644
ACGATAG11550.054.238125
GATAGTC14450.053.767367
ACGATAA15150.053.1310965
TATAGCG9650.052.9491657
TGATAGG28850.052.8900836
CGATAAT15250.052.553216
Produced by FastQC (version 0.11.5)