FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-40_HPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-40_HPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719300
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC2800.063.74965
CGCTACC2900.062.7582246
TCCCGTC13900.062.6951379
TGATAGG36100.060.9829946
CCCGTCC15750.060.66293710
GATAGGA44350.060.369447
GCGATAA10050.059.5518655
CTAGGAC11900.059.4092068
CTATAGG13100.059.045436
CGATAAC5250.057.9996386
GGTCCCC1750.057.9975057
CGGATAG10950.057.853525
GGATAGG17600.057.471236
GCGGTAC1950.057.4355355
CGCTACA3050.057.3766866
GCTGATA22400.057.343394
CCGATAA7450.056.845285
CGATAAA15100.056.787726
GGGTCCC3400.056.617296
CGCGATA7550.056.5559354
Produced by FastQC (version 0.11.5)