FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828402
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG9150.059.2880445
CGATAGT11200.058.4359476
GGATAGT18650.057.6124156
CGCGATA5600.056.873494
ACGATAG8500.056.822025
GATAGGA24850.056.474387
GATAGTC10000.056.3455167
GCGATAG11050.056.0618635
TGATAGG23700.055.9689836
CGGGATA9600.055.050634
GCGGTAC2100.054.998545
CAGATAG14000.054.998545
GATAGGT19900.054.870017
GATAGTG22050.054.2813957
CGATATG13750.053.9622086
CCGATAA8050.053.9116135
CGATAGG10500.053.665246
GCGATAA11050.053.5279925
TATAGCG8150.053.2472957
GCCGATA6400.053.0454674
Produced by FastQC (version 0.11.5)