FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916049
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGTC6400.056.8682569
GGATAGG19450.056.8570146
CCCGTCC7350.056.65994610
CGCGATA5500.055.993364
GGATAGT23050.055.26466
GGGATAG18400.054.585925
CGGATAG10350.054.099875
GATAGTC11450.053.7927477
CGATAGT12900.053.714566
CGGGATA7550.053.3049434
GATAGGG21050.053.0340927
GATAGGA27050.052.7848667
TGATAGG27450.052.6531376
GCGATAG10250.052.579135
GGTCCCC1600.052.4937747
CGCTACC2400.052.4937746
CAGATAG13950.052.4310575
GCGATAA10000.052.143825
GATAGGT23500.051.972567
CCGCTAC2900.051.89045
Produced by FastQC (version 0.11.5)