FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616557
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATAG13000.062.7253725
GCGATAA8650.061.4976045
CGGATAG11000.060.76755
TGATAGG23000.060.2556886
GATAGGA26800.059.6754237
GGATAGC6150.059.18196
GGTCCCC1600.059.055167
CGCGATA6000.058.91164
CGATAGG11250.058.7949456
ACGATAG8700.058.3283165
GATAGGG18550.058.2946437
CCTATAG6750.058.0690775
GATAGGT21400.057.399417
TGCGATA9550.057.167864
CGATAAT10100.056.8267946
GGGTCCC3450.056.8067136
CTATAGG13600.056.355456
GATAGTG15550.056.2631077
CCGATAA8150.056.2528345
CGGGATA8800.056.0747224
Produced by FastQC (version 0.11.5)