FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049010
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT13800.057.060996
CGCGATA6050.056.1115464
GATAGGT25400.056.078527
CGGGATA9650.055.850794
ACGATAG8600.055.3447345
CAGATAG13850.054.833485
GGATAGT24200.054.809996
CGGATAG9200.054.3981485
GGATAGG22000.053.450596
CCGCTAC2750.053.450595
TGATAGG26600.053.153966
GCGATAG11900.052.9372565
GATAGTC11650.052.8716247
GCGATAA10300.052.6660045
CCGATAG5750.051.73535
GATAGGG21850.051.4149557
CCTATAG5900.051.0131725
CTGATAG15600.050.9257165
GGGATAG20650.050.6742135
GCCGATA6650.050.5225754
Produced by FastQC (version 0.11.5)