FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356996
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG8300.059.4562265
CAGATAG11400.059.2527925
GATAGTC9600.058.3305247
CGGGATA9200.058.2049524
CGCGATA5450.057.1544234
GGATAGT15900.055.9116256
CGCTACA3100.054.1932376
CGGCTAC3300.054.0894475
CGATATG10000.053.549696
CTGATAG13100.053.4336745
TGATAGG16350.053.3024446
GCCGATA5400.053.146714
ACGATAG7450.053.085815
GCGATAA9800.052.8557175
CCGATAA6700.052.2373965
GATAGGT16750.051.8183987
CGATAGT9200.051.7388346
GATAGGG15450.051.6487
CGATAGG8200.051.646046
AGGATAG10850.051.611515
Produced by FastQC (version 0.11.5)