FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717071
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG19900.060.8499686
GATAGGA35200.060.7485477
TGATAGG30000.060.1957446
CGGATAG8150.059.689035
GCTACCG4800.059.0583237
GCGCTAC1900.058.94325
CCGCTAC1850.058.64455
CTAGGAC9000.058.327068
CGCGATA4700.057.336374
GATAGGT18650.057.2345627
CGCTACA3550.057.1790546
TCCCGTC6900.056.8054859
CGCTACG2250.055.996046
GGCTACC2250.055.996046
TATAGCG7250.055.9960377
CAGATAG11050.055.7426645
CCCGTCC8050.055.64619410
GCTCCCG4850.055.563087
GATAGGG19650.055.3904847
GGGATAG16200.055.3047335
Produced by FastQC (version 0.11.5)