FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPHK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPHK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115533
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG18000.060.082485
CGCGATA3950.059.3662454
GATAGGA24000.059.2074937
TGATAGG22750.057.2299586
CCTGATA8200.057.1943134
CGGATAG7550.057.0190545
CCGATAA6700.056.9394875
GCTGATA16450.056.8077054
GATAGTC8750.056.7991947
GTGATAG12950.056.2154245
GCGATAG7250.055.9992075
GCTAGCG1650.055.1507347
CCGTCCG700.055.0018231
GCGGTAC1400.054.999225
ACGATAG6200.054.757295
CGCTAGC1800.054.4436726
CTAGGAC6750.054.439818
CGATATG9650.054.403376
GCGATAA6900.054.2745935
GCTAGCC1550.054.1927837
Produced by FastQC (version 0.11.5)