FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPHK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPHK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872933
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA4850.056.285414
GGATAGT22150.055.933576
GGGATAG18700.055.585035
CGGATAG10850.055.4806675
GGATAGG22200.055.3346446
CGGGATA9500.054.891574
GCGATAA8550.054.8506365
CCTATAG5900.054.5731245
CGTATAG7500.054.130215
GATAGTC10900.053.9418457
GATAGGG22600.053.426127
CCGATAC1900.053.4179735
GATAGGT23450.052.683537
CTATAGG11850.052.2754676
ACGATAG8650.052.193525
CAGATAG14250.051.5759745
CGATAGT10800.051.5248076
GCGATAG10000.051.4470335
TATAGCG14650.050.6455467
TGATAGG28050.050.2823036
Produced by FastQC (version 0.11.5)