FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPHK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPHK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757024
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA6350.058.420794
GGATAGT17450.056.75796
CGGGATA9450.056.662394
CGGATAG10200.056.2702675
GATAGGT19100.055.3361437
CGCTAGC3500.054.9958576
CGCGGTC1400.054.9958534
TGATAGG20750.054.646486
GCGATAA10850.054.5120165
GATAGGA22250.054.265577
GGATAGG21050.054.200196
GATAGTG20650.054.0637257
AGGATAG13300.053.9433065
GATAGGG18600.053.4368327
CCCGATA4200.053.329314
CCCGTCC4750.053.04767210
CTGATAG14350.052.9228445
CGATAGT12300.052.638316
CAGATAG13150.052.163375
GCGATAG10300.051.9863745
Produced by FastQC (version 0.11.5)