FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPHK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPHK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542720
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC11500.059.34177410
TCCCGTC10150.059.30439
CGGATAG8700.059.133065
CAGATAG12750.058.1912885
GGATAGG17000.058.0551876
GATAGGA34300.057.6483157
CGTATAC2250.057.550825
TGATAGG30000.057.5130656
GTAGGAC9000.057.160848
CCTATAG5850.056.8329285
GATAGGT17350.056.0760777
GATAGGG18150.055.7254377
CCGCTAC2450.055.7096985
CGCGATA5050.055.4409834
GATAGTC11250.054.128887
CTAGGAC8750.053.994498
GCGATAA9550.053.869915
GGATAGT16700.053.8589026
GGTCCCG4000.053.3706057
GGGATAG15500.053.0601465
Produced by FastQC (version 0.11.5)