FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPHK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPHK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423603
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTC9400.061.7999887
CAGATAG12150.058.181275
CGCGATA6800.058.1537444
GGATAGT15850.057.184546
CGGATAG10000.057.042135
CGATAGG9700.057.002456
CGATAGT8100.056.1650926
GTGATAG16150.055.6889155
CGTATAG7850.054.83325
ACGATAG8300.054.8117145
CCGATAA7200.054.4369355
GATAGGA22050.054.278237
CGCTAAC2600.053.838736
CGGGATA8200.053.7730674
CCTGATA6400.053.586364
GTATAGG11850.053.4525456
GATAGTG16500.053.4471747
TGATAGG18350.053.398636
GCTGATA14850.053.2586444
GATAGGG16350.052.8673257
Produced by FastQC (version 0.11.5)